Harpagide Increases microRNA-140-5p Expression to inhibit Oxidised Low-Density Lipoprotein-Caused Human Umbilical Vascular Endothelial Cell Damage
نویسندگان
چکیده
To investigate the influence of harpagide isolated from Scrophularia ningpoensis Hemsl. on human umbilical vascular endothelial cells damage induced by oxidized low-density lipoprotein and its possible mechanism. Human were cultured in vitro. Different doses (20, 40, 80 μmol/l) applied to treat lipoprotein, overexpressing microRNA-140-5p μg/ml was with downregulation. Enzyme-linked immunosorbent assay kits used detect malondialdehyde content as well superoxide dismutase glutathione peroxidase activities. Flow cytometry Western blot utilized cell apoptosis. Ribonucleic acid expression analyzed real-time quantitative reverse transcription polymerase chain reaction. reduced rate apoptosis lipoprotein-stimulated (p<0.05), while elevated activities (p<0.05). MicroRNA-140- 5p overexpression lipoproteinstimulated Harpagide promoted after stimulation MicroRNA- 140-5p knockdown reversed inhibitory effect up-regulates inhibit oxidative stress lipoprotein-induced cells.
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ژورنال
عنوان ژورنال: Indian Journal of Pharmaceutical Sciences
سال: 2023
ISSN: ['0250-474X', '1998-3743']
DOI: https://doi.org/10.36468/pharmaceutical-sciences.1174